{"id":27,"date":"2026-03-11T14:11:00","date_gmt":"2026-03-11T14:11:00","guid":{"rendered":"https:\/\/site.uvm.edu\/alca\/?page_id=27"},"modified":"2026-07-01T13:35:26","modified_gmt":"2026-07-01T13:35:26","slug":"cary-eclipse-fluorescence-spectrometer","status":"publish","type":"page","link":"https:\/\/site.uvm.edu\/alca\/instrumentation\/cary-eclipse-fluorescence-spectrometer\/","title":{"rendered":"Fluorescence Spectrometer"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">The Cary Eclipse Fluorometer is a versatile fluorescence spectrophotometer designed for sensitive and precise measurement of fluorescence in a wide range of samples. Manufactured by Agilent Technologies, this instrument features advanced optics and detection systems that enable rapid scan speeds and exceptional signal-to-noise ratios, making it ideal for both routine and research applications.<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-adc57f8e wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading has-primary-uvmgreen-color has-text-color has-link-color wp-elements-2a24d65fc87da6d912a399adf2a747af\">Modes of Operation<\/h2>\n<\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\">Fluorescence <\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Measurement of fluorescence emission following excitation at a defined wavelength<\/li>\n\n\n\n<li>Supports:\n<ul class=\"wp-block-list\">\n<li>Emission scans<\/li>\n\n\n\n<li>Excitation scans<\/li>\n\n\n\n<li>Synchronous scanning<\/li>\n\n\n\n<li>Excitation &#8211; Emission Matrix (EEM) acquisition<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Phosphorescence<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Detection of long-lived luminescence following excitation<\/li>\n\n\n\n<li>Time-gated detection minimizes fluorescence background and scattered light interference<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Chemiluminescence<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Detection of light generated directly from chemical reactions without external excitation<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Bioluminescence<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Measurement of enzyme-driven luminescent systems such as luciferase-based assays<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading has-text-align-center has-primary-uvmgreen-color has-text-color has-link-color wp-elements-e35a208a7405c93d47b002973c984521\">Key Specifications <\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Excitation:<\/strong>&nbsp;200 -900 nm<\/li>\n\n\n\n<li><strong>Emission:&nbsp;<\/strong>200-900 nm<\/li>\n\n\n\n<li><strong>Spectral Bandwith<\/strong>: 1.5 \u2013 20 nm<\/li>\n\n\n\n<li><strong>Light Source<\/strong>: Xenon Flash Lamp&nbsp;<\/li>\n\n\n\n<li><strong>Maximum Scanning Speed<\/strong>: 24,000 nm\/min<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading has-text-align-center has-primary-uvmgreen-color has-text-color has-link-color wp-elements-9fd0d8ccdf4e0eb78ec469f9321e7331\">Available Accessories<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Multicell Holder&nbsp;<\/strong>\u2013 Ability to hold 4 cuvettes at once&nbsp;<\/li>\n\n\n\n<li><strong>Solid Sample Holder<\/strong>&nbsp;\u2013 can acquire spectra of many sample types including filters, powders, gels, optical components and fabrics.<\/li>\n\n\n\n<li><strong>Temperature Probes<\/strong>&nbsp;\u2013 accurately measure the temperature of the actual sample within the cuvette.&nbsp;<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-center has-primary-uvmgreen-color has-text-color has-link-color wp-elements-c8fbac6a0430be5db7081b773c64157b\">Future Capability Development&nbsp;<\/h2>\n\n\n\n<p class=\"has-text-align-left wp-block-paragraph\">The facility is evaluating the addition of a&nbsp;<strong>96-well plate reader accessory<\/strong>&nbsp;for higher-throughput fluorescence measurements. Users with interest in plate-based workflows are encouraged to reach out, as demand will help guide future instrumentation upgrades.<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-center has-primary-uvmgreen-color has-text-color has-link-color wp-elements-4c85efac5d18e729f8830f671bdb74b4\">Rates<\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes is-style-stripes--1\"><table class=\"has-fixed-layout\"><thead><tr><th class=\"has-text-align-center\" data-align=\"center\">Service (per hour)<\/th><th class=\"has-text-align-center\" data-align=\"center\">UVM Users<\/th><th class=\"has-text-align-center\" data-align=\"center\">External Users<\/th><\/tr><\/thead><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\">User Instrument Time<\/td><td class=\"has-text-align-center\" data-align=\"center\">$13.80<\/td><td class=\"has-text-align-center\" data-align=\"center\">$20.70<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">Staff Assisted Use<\/td><td class=\"has-text-align-center\" data-align=\"center\">$83.07<\/td><td class=\"has-text-align-center\" data-align=\"center\">$124.60<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">Training Session<\/td><td class=\"has-text-align-center\" data-align=\"center\">$83.07<\/td><td class=\"has-text-align-center\" data-align=\"center\">$124.60<\/td><\/tr><\/tbody><tfoot><tr><td class=\"has-text-align-center\" data-align=\"center\">Prices as of July 1, 2026<\/td><td class=\"has-text-align-center\" data-align=\"center\">&nbsp;<\/td><td class=\"has-text-align-center\" data-align=\"center\">&nbsp;<\/td><\/tr><\/tfoot><\/table><\/figure>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-adc57f8e wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h3 class=\"wp-block-heading has-text-align-center has-primary-uvmgreen-color has-text-color has-link-color wp-elements-fbc6f45e5064b4a755f9026bba77c833\">Training<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Interested in operating the fluorimeter independently?<\/strong><br>ALCA offers hands-on training covering instrument operation, method setup, detector optimization, and data acquisition for fluorescence, phosphorescence, chemiluminescence, and bioluminescence measurements.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h3 class=\"wp-block-heading has-text-align-center has-primary-uvmgreen-color has-text-color has-link-color wp-elements-81cf02e4f1bc8ebb8729caa940f0a538\">Analysis<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Need fluorescence or luminescence measurements performed by ALCA staff?<\/strong><br>Submit samples for full-service analysis, including experimental design, method development, data acquisition, and interpretation for solution-phase fluorescence and luminescence workflows.<\/p>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-50\"><a class=\"wp-block-button__link has-white-color has-primary-uvmgreen-background-color has-text-color has-background has-link-color wp-element-button\" href=\"https:\/\/site.uvm.edu\/alca\/contact\/\">Schedule Training<\/a><\/div>\n\n\n\n<div class=\"wp-block-button has-custom-width wp-block-button__width-50\"><a class=\"wp-block-button__link has-primary-uvmgreen-background-color has-background wp-element-button\" href=\"https:\/\/uvm.ilab.agilent.com\/service_center\/show_external\/6442?name=laboratory-for-chemical-analysis\">Request Analysis on iLabs<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Cary Eclipse Fluorometer is a versatile fluorescence spectrophotometer designed for sensitive and precise measurement of fluorescence in a wide range of samples. Manufactured by Agilent Technologies, this instrument features advanced optics and detection systems that enable rapid scan speeds and exceptional signal-to-noise ratios, making it ideal for both routine and research applications. Modes of [&hellip;]<\/p>\n","protected":false},"author":10857,"featured_media":25,"parent":20,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-27","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/site.uvm.edu\/alca\/wp-json\/wp\/v2\/pages\/27","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/site.uvm.edu\/alca\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/site.uvm.edu\/alca\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/site.uvm.edu\/alca\/wp-json\/wp\/v2\/users\/10857"}],"replies":[{"embeddable":true,"href":"https:\/\/site.uvm.edu\/alca\/wp-json\/wp\/v2\/comments?post=27"}],"version-history":[{"count":39,"href":"https:\/\/site.uvm.edu\/alca\/wp-json\/wp\/v2\/pages\/27\/revisions"}],"predecessor-version":[{"id":466,"href":"https:\/\/site.uvm.edu\/alca\/wp-json\/wp\/v2\/pages\/27\/revisions\/466"}],"up":[{"embeddable":true,"href":"https:\/\/site.uvm.edu\/alca\/wp-json\/wp\/v2\/pages\/20"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/site.uvm.edu\/alca\/wp-json\/wp\/v2\/media\/25"}],"wp:attachment":[{"href":"https:\/\/site.uvm.edu\/alca\/wp-json\/wp\/v2\/media?parent=27"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}