The Cary Eclipse Fluorometer is a versatile fluorescence spectrophotometer designed for sensitive and precise measurement of fluorescence in a wide range of samples. Manufactured by Agilent Technologies, this instrument features advanced optics and detection systems that enable rapid scan speeds and exceptional signal-to-noise ratios, making it ideal for both routine and research applications.
Modes of Operation
Fluorescence
- Measurement of fluorescence emission following excitation at a defined wavelength
- Supports:
- Emission scans
- Excitation scans
- Synchronous scanning
- Excitation – Emission Matrix (EEM) acquisition
Phosphorescence
- Detection of long-lived luminescence following excitation
- Time-gated detection minimizes fluorescence background and scattered light interference
Chemiluminescence
- Detection of light generated directly from chemical reactions without external excitation
Bioluminescence
- Measurement of enzyme-driven luminescent systems such as luciferase-based assays
Key Specifications
- Excitation: 200 -900 nm
- Emission: 200-900 nm
- Spectral Bandwith: 1.5 – 20 nm
- Light Source: Xenon Flash Lamp
- Maximum Scanning Speed: 24,000 nm/min
Available Accessories
- Multicell Holder – Ability to hold 4 cuvettes at once
- Solid Sample Holder – can acquire spectra of many sample types including filters, powders, gels, optical components and fabrics.
- Temperature Probes – accurately measure the temperature of the actual sample within the cuvette.
Future Capability Development
The facility is evaluating the addition of a 96-well plate reader accessory for higher-throughput fluorescence measurements. Users with interest in plate-based workflows are encouraged to reach out, as demand will help guide future instrumentation upgrades.
Rates
| Service (per hour) | UVM Users | External Users |
|---|---|---|
| User Instrument Time | $13.80 | $20.70 |
| Staff Assisted Use | $83.07 | $124.60 |
| Training Session | $83.07 | $124.60 |
| Prices as of July 1, 2026 |
Training
Interested in operating the fluorimeter independently?
ALCA offers hands-on training covering instrument operation, method setup, detector optimization, and data acquisition for fluorescence, phosphorescence, chemiluminescence, and bioluminescence measurements.
Analysis
Need fluorescence or luminescence measurements performed by ALCA staff?
Submit samples for full-service analysis, including experimental design, method development, data acquisition, and interpretation for solution-phase fluorescence and luminescence workflows.